Gene description for RAC1
Gene name Rac family small GTPase 1
Gene symbol RAC1
Other names/aliases RAC2
Species Canis lupus familiaris
 Experiment description of studies that identified RAC1 in Kidney cells

1
Experiment ID521
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23645497   
OrganismCanis lupus familiaris
Experiment descriptionOncogenic H-Ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition.
AuthorsTauro BJ, Mathias RA, Greening DW, Gopal SK, Ji H, Kapp EA, Coleman BM, Hill AF, Kusebauch U, Hallows JL, Shteynberg D, Moritz RL, Zhu HJ, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2013
SampleKidney cells
Sample nameNormal-Madin-Darby canine kidney cell (MDCK)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130021: EV-METRIC:50%

2
Experiment ID522
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23645497   
OrganismCanis lupus familiaris
Experiment descriptionOncogenic H-Ras reprograms Madin-Darby canine kidney (MDCK) cell-derived exosomal proteins following epithelial-mesenchymal transition.
AuthorsTauro BJ, Mathias RA, Greening DW, Gopal SK, Ji H, Kapp EA, Coleman BM, Hill AF, Kusebauch U, Hallows JL, Shteynberg D, Moritz RL, Zhu HJ, Simpson RJ.
Journal name Mol Cell Proteomics
Publication year2013
SampleKidney cells
Sample nameRas-transformed MDCK cells (21D1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130021: EV-METRIC:50%

3
Experiment ID1547
Identified moleculeProtein
Extracellular vesicle typeMicrovesicles
MISEVLarge EVs
Identification method Mass spectrometry
PubMed ID 33638284   
OrganismCanis lupus familiaris
Experiment descriptionTransglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition
AuthorsShafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ.
Journal name Proteomics
Publication year2021
SampleKidney cells
Sample nameMDCK - 10K pellet
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

4
Experiment ID1547
Identified moleculeProtein
Extracellular vesicle typeMicrovesicles
MISEVLarge EVs
Identification method Mass spectrometry
PubMed ID 33638284   
OrganismCanis lupus familiaris
Experiment descriptionTransglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition
AuthorsShafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ.
Journal name Proteomics
Publication year2021
SampleKidney cells
Sample nameMDCK - 10K pellet
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

5
Experiment ID1549
Identified moleculeProtein
Extracellular vesicle typeMicrovesicles
MISEVLarge EVs
Identification method Mass spectrometry
PubMed ID 33638284   
OrganismCanis lupus familiaris
Experiment descriptionTransglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition
AuthorsShafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ.
Journal name Proteomics
Publication year2021
SampleKidney cells
Sample nameMDCK (21D1) - 10K pellet
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

6
Experiment ID1549
Identified moleculeProtein
Extracellular vesicle typeMicrovesicles
MISEVLarge EVs
Identification method Mass spectrometry
PubMed ID 33638284   
OrganismCanis lupus familiaris
Experiment descriptionTransglutaminase-2, RNA-binding proteins and mitochondrial proteins selectively traffic to MDCK cell-derived microvesicles following H-Ras-induced epithelial-mesenchymal transition
AuthorsShafiq A, Suwakulsiri W, Rai A, Chen M, Greening DW, Zhu HJ, Xu R, Simpson RJ.
Journal name Proteomics
Publication year2021
SampleKidney cells
Sample nameMDCK (21D1) - 10K pellet
Isolation/purification methodsDifferential centrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

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